| 1. | Melanocyte stimulating hormone releasing factor , mrf
促黑激素释放因子 |
| 2. | Lrf luteinizing hormone releasing factor
黄体生成激素释放因子即 |
| 3. | Crf corticotropin releasing factor
促肾上腺皮质激素释放因子 |
| 4. | Corticotropin releasing factor
促肾上腺皮质激素释放因子 |
| 5. | Prolactin releasing factor , prf
催乳素释放因子 |
| 6. | Extro growth hormone releasing factor mediated by protoplast could more than 28 days retention in rabbit and mouse muscle that is condition of long - time expression of grf
通过提取注射部位dna后进行pcr鉴定，可以确认原生质体介导的grf基因至少可以在受试动物体内滞留28天以上，为grf的长期表达提供了条件。 |
| 7. | The result of investigation showing : extro growth hormone releasing factor mediated by protoplast expressed in rabbit muscle and mouse muscle , products of expression were detected by pcr rt - pcr and elisa
研究结果表明，以原生质体介导的外源性grf基因可以在家兔和小鼠肌肉中表达，通过pcr 、 rt - pcr和elisa都可以检测到表达产物。 |
| 8. | Growth hormone releasing factor is polypeptide hormone releasing by animal hypothaiami . it can induction synthesis and excretion of growth hormone which rise growth hormone level of animal . we large - scale cultured jm - 109 strain recombinant plasmid of grf
生长激素释放因子( growthhormonereleasingfactor ， grf )是由动物下丘脑合成并分泌的一种多肽激素，能特异性的诱导生长激素的合成与分泌，提高体内生长激素的水平。 |
| 9. | Il - 2 modulates the release of several hypothalamic and pituitary peptides , such as met - enkephalin , 9 - endorphin , prolactin , luteinizing hormone , follicle - stimulating hormone , thyrotropic hormone , growth hormone , adrenocorticotropic hormone , corticotropin releasing factor , arginine vasopressin and somatostain
Il - 2通过影响下丘脑-垂体-肾上腺皮质轴的功能，而调节多种下丘脑垂体激素和神经递质的释放，如加压素、乙酰胆碱和去甲肾上腺素等。 |
| 10. | Then the plasmid was extracted from them and determined by dna calculator . the protoplast that contain growth hormone releasing factor injected into rabbit muscle and mouse muscle after it were treated by 1 % glutaral , pay it to electric stimulation in muscle of injection site and extract omni - rna from injection site of rabbit muscle , expression of grf were detected by reverse transcription and pcr ; ratio of expression of grf were detected by elisa . extract dna form injection site of mouse muscle to research time of expression
本实验是将含grf重组质粒的jm109菌株大量培养，用碱裂变法提取质粒，用dnacaculator定量；制备含grf的原生质体，经1的戊二醛处理后注射于家兔肌肉，在注射部位给予电刺激，提取总rna ，用rt - pcr检测grf在肌肉中的表达；用elisa法定性检测grf在肌肉中蛋白质水平的表达；将该原生质体注射于小鼠肌肉中，定期提取dna ，初步探讨原生质介导的外源性grf在小鼠肌肉中的表达时间。 |
